Pour plate and spread plate method pdf

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pour plate and spread plate method pdf

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Aseptic Laboratory Techniques: Plating Methods

Spread plate technique is the method of isolation and enumeration of microorganisms in a mixed culture and distributing it evenly. The technique makes it easier to quantify bacteria in a solution. The spread plate technique involves using a sterilized spreader with a smooth surface made of metal or glass to apply a small amount of bacteria suspended in a solution over a plate. The plate needs to be dry and at room temperature so that the agar can absorb the bacteria more readily. A successful spread plate will have a countable number of isolated bacterial colonies evenly distributed on the plate. Save my name and email in this browser for the next time I comment.

Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Molten cooled agar approx. Microorganisms will grow both on the surface and within the medium. Colonies that grow within the medium generally are small in size and may be confluent; the few that grow on the agar surface are of the same size and appearance as those on a streak plate. Each both large and small colony is carefully counted using magnifying colony counter if needed. The number of microorganisms present in the particular test sample is determined using the formula:. To insure a countable plate a series of dilutions should be plated.

Skip to main content. Search form Search. Spread plate method. Figure 2. The design method is based on the recent research of Shentu, Jiang and Hsu. After incubation, the number of colonies present on the agar surface are counted, and the number of CFU in the sample is then calculated. Gram-positive using well diffusion method.

Pour plate Method: Principle, Procedure, Uses, and (Dis) Advantages

For its higher productivity, R2A medium should be recommended for heterotrophic plate counts in natural mineral water. This is a preview of subscription content, access via your institution. Rent this article via DeepDyve. Cavalli-Sforza, L. Analisi statistica per medici e biologi. Torino Italy: Editore Boringhieri.

Microorganisms are present on all inanimate surfaces creating ubiquitous sources of possible contamination in the laboratory. Experimental success relies on the ability of a scientist to sterilize work surfaces and equipment as well as prevent contact of sterile instruments and solutions with non-sterile surfaces. Here we present the steps for several plating methods routinely used in the laboratory to isolate, propagate, or enumerate microorganisms such as bacteria and phage. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials. Procedures described include 1 streak-plating bacterial cultures to isolate single colonies, 2 pour-plating and 3 spread-plating to enumerate viable bacterial colonies, 4 soft agar overlays to isolate phage and enumerate plaques, and 5 replica-plating to transfer cells from one plate to another in an identical spatial pattern. These procedures can be performed at the laboratory bench, provided they involve non-pathogenic strains of microorganisms Biosafety Level 1, BSL

The heterotrophic plate count HPC , formerly known as the standard plate count , is a procedure for estimating the number of live, culturable heterotrophic bacteria in water and for measuring changes in swimming pools or during water treatment and distribution. Colonies may arise from pairs, chains, clusters, or single cells—all of which are included in the term colony-forming units CFU. The final count also depends on interaction among developing colonies. Choose the procedure and medium that best suit how the resulting information will be used. Only compare data generated using the same procedure and medium. Four methods and five media are described.

Pour plate Method: Principle, Procedure, Uses, and (Dis) Advantages

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Spread Plate Technique- Principle, Procedure and Uses

Spread Plate Technique- Principle, Procedure and Uses

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