Equipment found in microbiology laboratory and their uses pdf

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equipment found in microbiology laboratory and their uses pdf

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List of equipment / apparatus used in microbiology laboratory

Autoclave 2. Incubator 3. Hot air oven 4. Inoculating loop 5. Water bath 7. Heating mantle 8. Hot plate with magnetic stirrer 9. UV chamber Inoculation chamber Colony counter Microscope Refrigerator Bunsen burner Spirit lamp Micrometer stage and ocular Balance Digital and 4-beam Thermometer Autoclave It is a robust, electrically heated steam vessel meant for sterilizing thermostable culture media, glassware, and other materials that are not spoiled by moist heat.

Autoclave runs on the principle of pressure cooker. The moist heat steam has a very good penetrating power. In routine process, sterilization can be achieved by operating the autoclave at C 15 psig for 15 min. In its simplest form, the equipment has a removable lid for the delivery of materials to be sterilized. It is necessarily equipped with a gasket, pressure-cum-temperature gauge, a vent for letting out air or excess pressure, a safety valve, and a drain. The figure of a portable autoclave is given in Fig Incubator This an insulated, electrically heated cabinet meant for providing microorganisms with optimum temperature for growth.

The cabinet is insulated and thermostatically controlled. For routine purposes, the temperature is maintained at C for bacteria, about 25 C for molds, and C for mesophilic bacteria. A temperature as 1. A very common laboratory incubator is shown in Fig Hot air oven This is similar to incubator in make except that it can operate at temperatures up to C and has a fan for circulating hot air.

Hot air oven is used for sterilization of glassware and materials that are spoiled by moist heat. The death of cells occurs due to the oxidation of cellular constituents by the dry heat. For routine purpose, sterilization can be achieved by running the equipment at C for 1.

Hot air oven is less effective than autoclave. Inoculating loop This is a tool for transferring and streaking cultures. It consists of a thin nichrome wire whose one end is twisted into a small loop while the other end is fixed to a thermoset plastic handle. Sometimes, the looped end is 2. Inoculating needles are used for preparing stab cultures. Vortex mixer This equipment is used for mixing liquids kept in a test tube. It has one or more cup-like depressions at the top to receive the bottom of the test tube.

The machine is electrically powered. When actuated, the machine moves the bottom of the test tube in a gyratory motion, thereby affecting a thorough mixing of the solution. The speed of the mixer can be varied. It can also be used to maintain constant temperature that is required in microbiology lab work.

Several models and types of water bath are available. It is electrically heated and thermostatically controlled. Heating mantle It is an electrically heated and thermostatically controlled unit used to heat or melt samples and reagents. The inner lining is made of asbestos and therefore gives an indirect heat to the materials to be heated.

Hot plate with magnetic stirrer This is an electrically powered equipment performs the dual function of heating and agitation. The agitation occurs by magnetic arrangement.

Any type of glassware can be used for the heating and agitation. Magnetic beads are used for the agitation. The equipment has two lamps for long- and short wavelength UV radiation.

Since UV radiation is genotoxic mutagenic its exposure to skins and eyes must be avoided. A viewport with colored glass is provided for safety. The chamber is equipped with UV lamp for periodic disinfection of the chamber. While working, the UV light must be turned off and day-light bulb is turned on. In microbiology lab, it is used for maintaining ph of the medium and diluents.

The ph meter must be standardized with buffer solutions before operation. Since the instrument is very sensitive, it must not be used for stirring and it must not be dipped in hot or very cold solutions. The electrodes must always be kept immersed in suitable solutions. Read the manual carefully before using the instrument. Colony counter It is used for counting microbial colony bacterial and yeast. The instrument is equipped with a backlight source, gridlines and a magnifying lens.

It also has a sensor for digitally registering the number of colonies counted Fig. Microscope It is an instrument for observing microscopic items such as cells, crystals and cell organelles.

It has the dual function of magnification and resolution. For routine microbiological works, bright field compound microscope with oil immersion objective is adequate. A compound microscope is shown in Fig. Refrigerator This is a common household equipment for keeping foods and beverages cool.

The equipment is electrically powered and uses ammonia as the refrigerant. Bunsen burner Bunsen burner is a common tool used in science lab Fig.

In microbiology lab, it is used for sterilizing inoculating loop, plating out cultures, transferring cultures, heat-fixing of smears and creating a sterile zone for aseptic operation.

Spirit lamp The function of spirit lamp is the same as the Bunsen burner but is portable. It uses rectified spirit as the fuel produces smoke-free flame. The lamp must be covered with a lid when not in use to prevent loss of spirit Fig. Micrometers stage and ocular These are graduated glass pieces Fig. Stage micrometer is a slide on which etching is done with mm spacing.

The ocular micrometer, which is place on the eyepiece, has an arbitrary scale and must be calibrated against the stage micrometer. During measurements, the ocular micrometer is retained while the stage micrometer is replace with the specimen slide.

Balance Balance is needed in microbiology lab for weighing chemicals, samples, media, etc. Digital balances are fast to work with but needs frequent calibration Fig. The triple-beam and 4-beam balances are robust equipment that need little care and maintenance. Beam balances run on mechanical principles while the principles on which electronic balances run is quite complicated Fig. Thermometer Thermometers are required to ensure the heating equipment is running at the correct temperature.

The temperature of the medium, incubator, etc. Mercury in glass thermometers are standard 9. Digital thermometers use probes for measurement of temperatures. Coliform membrane filter This glass equipment is used for the testing of coliforms in water Fig. The filter retains the microorganisms. The filter is then aseptically transferred to a selective-cum-differential semisolid medium kept in a petri dish.

If there are coliforms, they will appear as pink dots after incubation at 35 C for 22 hrs. Lab Exercise 3: Media, incubation, and aseptic technique Objectives 1. Compare the different types of media. Describe the different formats of media, plate, tube etc. Explain how to sterilize it,. To demonstrate skill in the use of ocular and stage micrometers for measurements of cell size. To recognize. Sterile Technique It is very important in microbiology to work with pure cultures.

Unfortunately this is difficult. The world around us is covered with microorganisms.


Laboratory services are provided in a variety of settings: physicians offices, clinics, hospitals, and regional and national referral centers. Laboratories used for scientific research take many forms because of the differing requirements of specialists in the various fields of science and engineering. A physics laboratory might contain a particle accelerator or vacuum chamber , while a metallurgy laboratory could have apparatus for casting or refining metals or for testing their strength. A chemist or biologist might use a wet laboratory , while a psychologist's laboratory might be a room with one-way mirrors and hidden cameras in which to observe behavior. In some laboratories, such as those commonly used by computer scientists , computers sometimes supercomputers are used for either simulations or the analysis of data. Scientists in other fields will use still other types of laboratories.

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Basic Equipment and Microbiological Techniques for Studying Bacterial Adhesion

Confirmation and maintenance of documents Instruction manuals, qualification protocol and certificates. As we proceed through the semester, hopefully the labs will fulfill these purposes. Welcome to Termaks AS.

29 Equipment’s Every Microbiology Laboratory Should Have

Handbook of Bacterial Adhesion pp Cite as. The knowledge of laboratory safety, major equipment, and basic microbiological techniques are essential for conducting studies on bacterial adhesion. This chapter is written for beginners in the field of microbiology and for nonmicrobiologists who want to set up a laboratory for conducting bacterial adhesion studies.

List of equipment / apparatus used in microbiology laboratory

In the laboratory, eight main types of instruments are used:- 1. Balance 2. Centrifug e 3.

A modern microbiology laboratory should be furnished with the following equipment. Liquid substances, such as prepared media and saline solutions cannot be sterilized in oven, as they lose water due to evaporation. An oven Figure 3. The thermostat dial reading is approximate and the exact temperature is read by introducing a thermometer into the oven or on a built-in L-shaped thermometer. In a modern oven Figure 3. Time is set by a digital timer.

Our quality for your lab! From sample preparation There is no contact between the sample and the machine to avoid cross-contamination. Images and results are automatically saved on your computer to ensure traceability. Sample preparation No risk of cross-contamination Accurate results and excellent reproducibility Significantly increases your analyzing capacities How to prepare your sample?

A very common laboratory incubator is shown in Fig. 2. 3. Hot air oven. This is similar to incubator in make except that it can operate at temperatures up to °​C.


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  • PDF | educational material | Find, read and cite all the research you need on ResearchGate. Autoclaves are used to sterilize equipment and supplies. by subjecting them to high Condensation occurs as long as there is a. Gianfranco N. - 02.06.2021 at 20:29
  • Autoclave 2. Kai P. - 06.06.2021 at 06:30
  • PDF | On Oct 10, , Abu Nayeem published An Assignment of Microbiology Laboratory Use of Microbiology Lab, Chemical and Equipment | Find, read and cite all the research you need on using a single-lens microscope of his own design. found in almost all orders of the class of Bacteria. Licinio S. - 06.06.2021 at 07:52